Adventitious shoot regeneration from leaf explants of eastern cottonwood (Populus deltoides) cultured under photoautotrophic conditions.
Identifieur interne : 003715 ( Main/Exploration ); précédent : 003714; suivant : 003716Adventitious shoot regeneration from leaf explants of eastern cottonwood (Populus deltoides) cultured under photoautotrophic conditions.
Auteurs : Marco Mingozzi [États-Unis] ; Paul Montello ; Scott MerkleSource :
- Tree physiology [ 0829-318X ] ; 2009.
Descripteurs français
- KwdFr :
- Dioxyde de carbone (métabolisme), Feuilles de plante (physiologie), Fructose (métabolisme), Glucose (métabolisme), Lumière (MeSH), Métabolisme glucidique (MeSH), Populus (croissance et développement), Populus (métabolisme), Pousses de plante (croissance et développement), Pousses de plante (métabolisme), Processus autotrophes (MeSH), Saccharose (métabolisme), Techniques de culture (MeSH).
- MESH :
- croissance et développement : Populus, Pousses de plante.
- métabolisme : Dioxyde de carbone, Fructose, Glucose, Populus, Pousses de plante, Saccharose.
- physiologie : Feuilles de plante.
- Lumière, Métabolisme glucidique, Processus autotrophes, Techniques de culture.
English descriptors
- KwdEn :
- Autotrophic Processes (MeSH), Carbohydrate Metabolism (MeSH), Carbon Dioxide (metabolism), Culture Techniques (MeSH), Fructose (metabolism), Glucose (metabolism), Light (MeSH), Plant Leaves (physiology), Plant Shoots (growth & development), Plant Shoots (metabolism), Populus (growth & development), Populus (metabolism), Sucrose (metabolism).
- MESH :
- chemical , metabolism : Carbon Dioxide, Fructose, Glucose, Sucrose.
- growth & development : Plant Shoots, Populus.
- metabolism : Plant Shoots, Populus.
- physiology : Plant Leaves.
- Autotrophic Processes, Carbohydrate Metabolism, Culture Techniques, Light.
Abstract
Effects of photoautotrophic and photomixotrophic growth conditions on adventitious shoot regeneration from leaf explants of eastern cottonwood (Populus deltoides Bartr. ex Marsh.) were investigated. Rooting and proliferating shoot cultures (Stage I) were grown in either an elevated (1500 ppm) CO(2) concentration ([CO(2)]) at high photosynthetic photon flux (PPF; ~ 150 micromol m(-2) s(-1)) (photoautotrophic condition) with 0, 10 or 30 g l(-1) sucrose or under standard conditions (ambient (360 ppm) [CO(2)] at low PPF (~ 60 micromol m(-2) s(-1)) with 30 g l(-1) sucrose). Leaves harvested from these cultures were analyzed for soluble sugars and were used as explants for adventitious shoot regeneration (Stage II), which was also carried out under photoautotrophic and standard conditions. Photoautotrophic conditions during Stage I promoted growth of rooting shoots but inhibited axillary shoot proliferation. Photoautotrophic conditions during Stage II suppressed callus and adventitious bud production from leaf explants compared with standard conditions. The regeneration environment appeared to be more important in controlling bud formation than the conditions under which the donor shoots were grown. Regardless of Stage I treatment, bud production was up to 100-fold higher for leaves cultured under standard conditions than under photoautotrophic conditions. Once adventitious buds were differentiated from the leaf tissues, however, their elongation was faster under photoautotrophic conditions than that under standard conditions, with some shoots reaching 10 mm in length on leaf explants cultured under photoautotrophic conditions. Because total leaf soluble sugar concentration was always lowest in shoots under standard conditions, which also yielded the highest bud production, the results suggest that endogenous starvation enhanced shoot production.
DOI: 10.1093/treephys/tpn029
PubMed: 19203957
Affiliations:
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(métabolisme)</term><term>Techniques de culture (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon Dioxide</term><term>Fructose</term><term>Glucose</term><term>Sucrose</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term><term>Pousses de plante</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dioxyde de carbone</term><term>Fructose</term><term>Glucose</term><term>Populus</term><term>Pousses de plante</term><term>Saccharose</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Feuilles de plante</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Autotrophic Processes</term><term>Carbohydrate Metabolism</term><term>Culture Techniques</term><term>Light</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Lumière</term><term>Métabolisme glucidique</term><term>Processus autotrophes</term><term>Techniques de culture</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Effects of photoautotrophic and photomixotrophic growth conditions on adventitious shoot regeneration from leaf explants of eastern cottonwood (Populus deltoides Bartr. ex Marsh.) were investigated. Rooting and proliferating shoot cultures (Stage I) were grown in either an elevated (1500 ppm) CO(2) concentration ([CO(2)]) at high photosynthetic photon flux (PPF; ~ 150 micromol m(-2) s(-1)) (photoautotrophic condition) with 0, 10 or 30 g l(-1) sucrose or under standard conditions (ambient (360 ppm) [CO(2)] at low PPF (~ 60 micromol m(-2) s(-1)) with 30 g l(-1) sucrose). Leaves harvested from these cultures were analyzed for soluble sugars and were used as explants for adventitious shoot regeneration (Stage II), which was also carried out under photoautotrophic and standard conditions. Photoautotrophic conditions during Stage I promoted growth of rooting shoots but inhibited axillary shoot proliferation. Photoautotrophic conditions during Stage II suppressed callus and adventitious bud production from leaf explants compared with standard conditions. The regeneration environment appeared to be more important in controlling bud formation than the conditions under which the donor shoots were grown. Regardless of Stage I treatment, bud production was up to 100-fold higher for leaves cultured under standard conditions than under photoautotrophic conditions. Once adventitious buds were differentiated from the leaf tissues, however, their elongation was faster under photoautotrophic conditions than that under standard conditions, with some shoots reaching 10 mm in length on leaf explants cultured under photoautotrophic conditions. Because total leaf soluble sugar concentration was always lowest in shoots under standard conditions, which also yielded the highest bud production, the results suggest that endogenous starvation enhanced shoot production.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19203957</PMID><DateCompleted><Year>2009</Year><Month>06</Month><Day>04</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0829-318X</ISSN><JournalIssue CitedMedium="Print"><Volume>29</Volume><Issue>3</Issue><PubDate><Year>2009</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Adventitious shoot regeneration from leaf explants of eastern cottonwood (Populus deltoides) cultured under photoautotrophic conditions.</ArticleTitle><Pagination><MedlinePgn>333-43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpn029</ELocationID><Abstract><AbstractText>Effects of photoautotrophic and photomixotrophic growth conditions on adventitious shoot regeneration from leaf explants of eastern cottonwood (Populus deltoides Bartr. ex Marsh.) were investigated. Rooting and proliferating shoot cultures (Stage I) were grown in either an elevated (1500 ppm) CO(2) concentration ([CO(2)]) at high photosynthetic photon flux (PPF; ~ 150 micromol m(-2) s(-1)) (photoautotrophic condition) with 0, 10 or 30 g l(-1) sucrose or under standard conditions (ambient (360 ppm) [CO(2)] at low PPF (~ 60 micromol m(-2) s(-1)) with 30 g l(-1) sucrose). Leaves harvested from these cultures were analyzed for soluble sugars and were used as explants for adventitious shoot regeneration (Stage II), which was also carried out under photoautotrophic and standard conditions. Photoautotrophic conditions during Stage I promoted growth of rooting shoots but inhibited axillary shoot proliferation. Photoautotrophic conditions during Stage II suppressed callus and adventitious bud production from leaf explants compared with standard conditions. The regeneration environment appeared to be more important in controlling bud formation than the conditions under which the donor shoots were grown. Regardless of Stage I treatment, bud production was up to 100-fold higher for leaves cultured under standard conditions than under photoautotrophic conditions. Once adventitious buds were differentiated from the leaf tissues, however, their elongation was faster under photoautotrophic conditions than that under standard conditions, with some shoots reaching 10 mm in length on leaf explants cultured under photoautotrophic conditions. Because total leaf soluble sugar concentration was always lowest in shoots under standard conditions, which also yielded the highest bud production, the results suggest that endogenous starvation enhanced shoot production.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mingozzi</LastName><ForeName>Marco</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Warnell School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602, USA. marco.ming@libero.it</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Montello</LastName><ForeName>Paul</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Merkle</LastName><ForeName>Scott</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>12</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>142M471B3J</RegistryNumber><NameOfSubstance UI="D002245">Carbon Dioxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>30237-26-4</RegistryNumber><NameOfSubstance UI="D005632">Fructose</NameOfSubstance></Chemical><Chemical><RegistryNumber>57-50-1</RegistryNumber><NameOfSubstance UI="D013395">Sucrose</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D052818" MajorTopicYN="Y">Autotrophic Processes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="Y">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002245" MajorTopicYN="N">Carbon Dioxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046508" MajorTopicYN="N">Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005632" MajorTopicYN="N">Fructose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008027" MajorTopicYN="N">Light</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013395" MajorTopicYN="N">Sucrose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>2</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>2</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>6</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19203957</ArticleId><ArticleId IdType="pii">tpn029</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpn029</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><noCountry><name sortKey="Merkle, Scott" sort="Merkle, Scott" uniqKey="Merkle S" first="Scott" last="Merkle">Scott Merkle</name><name sortKey="Montello, Paul" sort="Montello, Paul" uniqKey="Montello P" first="Paul" last="Montello">Paul Montello</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Mingozzi, Marco" sort="Mingozzi, Marco" uniqKey="Mingozzi M" first="Marco" last="Mingozzi">Marco Mingozzi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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